Studies on Nucleic Acid Biosynthesis in Ehrlich Ascites Cells Suspended in a Medium Permitting Growth
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چکیده
منابع مشابه
Nucleic acid metabolism in Ehrlich ascites tumor cells.
The incorporation of P32 into deoxyribonucleic acid (DNA) by Ehrlich ascites tumor cells has been found to be considerably more radioresistant when carried out in vitro than in vivo (6). To study the nature of the in vitro incorporation and explore possible reasons for its radioresistance, we have in vestigated the incorporation of P32 and various C'Mabeled precursors into the nucleic acids of ...
متن کاملGrowth-promoting effect of acid mucopolysaccharides on Ehrlich ascites tumor.
The effect of acid mucopolysaccharides on the growth of hypotetraploid Ehrlich ascites tumor was investigated using chondroitin sulfate and crude hyaluronic acid. Chondroitin sulfate solution was given s.c. into the right flank of SM mice, followed immediately by Ehrlich tumor. Average tumor weights in chondroitin sulfate-treated groups on Days 3 and 8 after tumor inoculation were 2-3 times as ...
متن کاملStudies on the mechanism of inhibition by polyenic antibiotics of nucleic acid biosynthesis in ascites tumor cells.
The polyenic antibiotic lucensomycin caused a severe inhibition of uridine or thymidine incorporation into RNA or DNA of ascites tumor cells. The inhibition appeared to be related to the ability of the polyene to interact with the cell membrane, the permeability of which was increased; simple leakage of precursors or of enzymes from the cells did not fully account for the kinetics of inhibition...
متن کاملStudies on simplified media for survival of Ehrlich ascites cells.
Maintenance of Ehrlich ascites tumor cells for periods up to several weeks (11, 15) has been ac complished by the use of media containing high concentrations of serum, embryo extract, or bovine amniotic fluid.1 Synthetic medium 199 devised by Morgan, Morton, and Parker (12) will permit sur vival for periods of at least a few days, as evi denced by the serial propagation of Mengo en cephalitis a...
متن کاملDeoxycytidylic acid deaminase in Ehrlich ascites tumor cells.
verts dCMP to dUMP (13, 19), is present in mammalian tissues and manifests a high activity in such rapidly pro liferating tissues as embryonic tissue or tumors (3, 13, 15, 19). In tissues with a low mitotic index, the activity of the enzyme is quite low. Thus dCMP-deaminase activity is low in adult rat liver but increases when cell proliferation occurs in the organ, as for example after hepatec...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1959
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)98168-8